Talk:20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3)
Agarose Gels
TR lab
Well folks, today we learned what happens when you switch from 10X to 50X concentrate of TAE buffer, and forget that you have done so! For posterity's sake, your gel images (with their fragments mostly run off) are below. For the For Next Time assignment, please do your calculations using the images from the Wednesday/Friday section.
Looks like those far-run bands are indeed what we were after. The purified products look good!
| Lane | Sample | Lane | Sample |
|---|---|---|---|
| 1 | Ladder | 8 | Yellow bkb |
| 2 | David bkb | 9 | Yellow frag |
| 3 | David frag | 10 | Green bkb |
| 4 | Purple bkb | 11 | Green frag |
| 5 | Purple frag | 12 | Red/Pink bkb |
| 6 | Blue bkb | 13 | Red/Pink frag |
| 7 | Blue frag |
The ladder amount loaded results in 125 ng of DNA for the 3 Kbp fragment. Further details about the ladder are here.
David's samples contain 3 μL of DNA. Approximately 20 μL per lane of your samples were loaded (which is how many μL of DNA?).
WF lab
All right, plenty of DNA today! If your ladder bands are difficult to see, go ahead and use someone else's picture to do the calculations for problem 1 of the For Next Time assignment.
For some reason the uncut backbone didn't show up too well today (presumably it was at a lower concentration than we thought), so for your your reference I've included an old gel I ran with pCX-NNX above. Uncut plasmid is in lanes 2 and 3.
| Lane | Sample | Lane | Sample |
|---|---|---|---|
| 1 | Ladder | 8 | Green bkb |
| 2 | Red bkb | 9 | Green frag |
| 3 | Red frag | 10 | Blue bkb |
| 4 | Pink bkb | 11 | Blue frag |
| 5 | Pink frag (no band) | 12 | Purple bkb |
| 6 | Yellow bkb | 13 | Purple frag (faint) |
| 7 | Yellow frag |
The ladder amount loaded results in 125 ng of DNA for the 3 Kbp fragment. Further details about the ladder are here. Approximately 22 μL per lane of your samples were loaded (which is how many μL of DNA?).
