Talk:20.109(S09): Transfection (Day5)
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T/R Luciferase Reactions
Be sure to indicate if any of your samples were diluted!
| Team Colour | Sample 1 | Sample 2 | Sample 3 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Firefly | Renilla | Ratio | Firefly | Renilla | Ratio | Firefly | Renilla | Ratio | |
| Red | .018 | .105 | 5.833 | .747 (diluted) | 731.3 | 979.0 | 1.012 | 335.2 | 331.2 |
| Orange | 0.102 | 0.601 | 5.9 | 4.0 (diluted 1:10) | 4660 (diluted 1:10) | 1167 | 13.1 | 3750 | 287 |
| Yellow | .058 | .512 | 8.82 | 5.122 (diluted 1:10) | 5515 (diluted 1:10) | 1076.73 | 14.52 | 4177 | 287 |
| Green | 733 | 766 | 1.04 | 7,345 | 7,253,398 | 987.5 | 2,695 | 727,637 | 270.0 |
| Blue | 459 | 587 | 1.28 | 7,179 | 7,796,501 | 1086 | 2,407 | 663,775 | 275.77 |
| Pink | .016 | .744 | 46.5 | 9.004(diluted 1:10) | 9607(diluted) | 1067(diluted) | 13.76 | 1738 | 126 |
| Purple | 359 | 477 | 1.33 | 5738 | 6919017 | 1206 | 2004 | 673580 | 336.1 |
| Grey | .006 | .094 | 15.66 | .918 (1:10) | 1107 | 1205.9 | .955 | 325.3 | 340.63 |
W/F Luciferase Reactions
Be sure to indicate if any of your samples were diluted!
| Team Colour | Sample 1 | Sample 2 | Sample 3 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Firefly | Renilla | Ratio | Firefly | Renilla | Ratio | Firefly | Renilla | Ratio | |
| Red | 0.135 | 0.766 | 5.674 | 3.161 | 2624 | 830.1 | 13.74 | 3521 | 256.25 |
| Orange | 417 | 429 | 1.028 | 6164 | 8362596 | 1356.8 | 1918 | 716605 | 374.5 |
| Yellow | 0.009 | 0.075 | 8.33 | 0.295 | 230.5 | 781.4 | 1.079 | 334.8 | 310.3 |
| Green | 320 | 361 | 1.128 | 4772 | 8946135 | 1874.714 | 2819 | 739.415 | 262.297 |
| Blue | 1413 | 1610 | 1.1394 | 7491 | 7534704 | 1005.834 | 3572 | 609682 | 170.684 |
| Pink | 0.187 | 0.813 | 4.348 | 53.29 | 58380 | 1095.52 | 14.71 | 4353 | 295.92 |
| Purple | 445 | 519 | 1.166 | 7063 | 7174730 | 1015.819 | 2242 | 487690 | 217.524 |
| Grey | 0.013 | 0.162 | 12.5 | 0.276 | 222.9 | 808 | 0.98 | 292.5 | 298 |
