Talk:20.109(S10):Induce protein and evaluate DNA (Day5)
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TR O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD M124S | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Red | 0.229 | 0.302 | 0.251 | All bright yellow-green (after O/N). [All pellets were slightly yellow. Pellet of wildtype had more green than the others. We left them to grow overnight] |
Orange | 0.227 (0.1713) | 0.257 (0.1823) | 0.263 (0.1624) | All bright yellow-green (after O/N). [Slightly green. Overnight at RT.] |
Yellow | 0.232 (.152) | 0.244 (.151) | 0.266(.146) | All bright yellow-green (after O/N). [All pellets were slightly yellow, but we decided to place them in the shaker at room temp. overnight.] |
Green | 0.225 | 0.235 | 0.226 | All bright yellow-green (after O/N). |
Blue | 0.231 (0.169) | 0.271 (0.205) | 0.218 | All bright yellow-green (after O/N). [Slightly yellow except for M124S sample] |
Pink | 0.215 | 0.266 | 0.210 | All bright yellow-green (after O/N). |
Purple | 0.243 (0.144) | 0.237 (0.148) | 0.183 (0.105) | All bright yellow-green (after O/N). [Due to the imperfect (sadly) nature of our pellet, we left the samples at room temperature shaker overnight. Also, regarding our gel, we placed the 1kbp ladder in lane 8, not lane 3.] |
Gels
WF O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD M124S | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Red | 0.206 | 0.283 | 0.206 | All bright yellow-green (after O/N). [Yes, except M124S is faint yellow so we decided to go overnight] |
Orange | 0.214 | 0.290 | 0.208 | All bright yellow-green (after O/N). [yes] |
Yellow | 0.249 | 0.237 | E67K2; OD = 0.232 | yes; they were yellow so we decided not to go overnight. |
Green | 0.238 | 0.273 | 0.214 | All bright yellow-green (after O/N). [NO] |
Blue | .281 | .296 | .242 | Yes |
Pink | 0.246 | 0.262 | 0.191 | The pellet in WT and E84G were bright yellow after 2 hours and 21 min. M124S was white-ish but was yellow after incubating an extra 15 minutes. |
Purple | .176 | .192 | .109 | Yes |
Gels