Talk:20.109(F07):Start-up genome engineering

From OpenWetWare

Jump to: navigation, search
schematic for oligonucleotide pair
oligo name oligo number oligo sequence primer record enzymes added with insert
TeacherOligo_top NO196 5' GAT CTG CGC ACG CGT teacher sample oligo record FspI and MluI
TeacherOligo_btm NO197 5'G,ATC,ACG,CGT,GCG,CA
M13KO7seq_2101_fwd NO198 5'- CAA GGC ACT GAC CCC GTT AAA ACT TAT TAC -3'
M13KO7seq_2151_fwd NO199 5'- AAA AGC CAT GTA TGA CGC TTA CTG GAA CGG -3'


T/R lab

oligo name oligo number oligo sequence primer record enzymes added with insert
TRpink_top NO202 5’ GAT CTC GAC GGC GCC 3’ Oligo design record Sal I
TRpink_btm NO203 5’ G ATC GGC GCC GTC GA 3’ Oligo design record Sfo I
TRyellow_top NO204 5' GAT CTA GAT ATC 3'
edit:5'gat cta gaC GAt atc
Yellow Insert Design Xba I, Eco RV
TRyellow_btm NO205 5' G ATC GAT ATC TA 3'
edit: 5'G atc gat atc gtc ta
Yellow Insert edits!
TRblue_top NO206 5' GAT CAG ATC TTA GGC GCC CGA 3' Blue Insert Design BglII
TRblue_btm NO207 5' G ATC TGC GGC GCC TAA GAT CT 3' Blue Insert Design SfoI
TRpurple_top NO208 5' GAT CTG ATC AGT ACT 3' Purple Insert Design BclI, ScaI
TRpurple_btm NO209 5' GAT CAG TAC TGA TCA 3'
TRred_top NO210 5' GAT CTT AAG GAC GTC Red Insert Design AflII ZraI
TRred_btm NO211 5' G ATC GAC GTC CTT AA
TRgreen_top NO212 5' GAT CGG TAC CAC GTC 3' Green Insert Design KpnI BmgI
TRgreen_btm NO213 5' G ATC GAC GTG GTA CC 3'

W/F lab

oligo name oligo number oligo sequence primer record enzymes added with insert
WFpink_top NO214 G A T , C A T , G A A , T T C, G A T , A T C pink insert design EcoRI, EcoRV
WFpink_btm NO215 G A T , C G A , T A T , C G A , A T T , C A T
WFyellow_top NO
WFyellow_btm NO
WFblue_top NO216 gatcagatctcccacgtc Blue Insert Design BglII,BmgBI
WFblue_btm NO217 gatcgacgtgggagatct
WFpurple_top NO218 gat/cGA/ATT/CAC/GTC Purple Insert Design EcoRI, BmgBI
WFpurple_btm NO219 g/atc/GAC/GTG/AAT/TC
WFred_top NO220 gat/cGA/ATT/CTT/GTT/AAC Red Insert Design EcoRI, HpaI
WFred_btm NO221 g/atc/GTT/AAC/AAG/AAT/TC
WFgreen_top (NO218) 5’-gat,cga,att,cac,gtc,gat,c-3’ Green Insert Design EcoRI, BmgBI
WFgreen_btm (NO219) 5’-g,atc,gac,gtg,aat,tcg,atc-3’

[[Media:Media:Example.oggMedia:Example.ogg]]

Personal tools