Talk:20.109(S07):Start-up genome engineering

From OpenWetWare

Jump to: navigation, search

Insert designs

T/R lab

protein to modify oligo name oligo number oligo sequence primer record enzyme for verification of insert
p8 TRpink_top NO TCT GAA CAG AAA CTG ATA TCT GAA GAA GAC CTG GAt gca pink oligo record AlwN1


5' CAG AAA \ CTG 3'
3' GTC \ TTT GAC 3'

p8 TRpink'_btm NO CCTAGGTCTTCTTCAGATATCAGTTTCTGTTCAGAtgca This restriction enzyme cuts our oligo once and the M13 twice.
p3 TRyellow'_top NO. 28488673 gat cTG GAA CAG AAA CTG ATA TCT GAA GAA GAC CTA yellow oligo record EcoRV
p3 TRyellow'_btm NO. 28488674 gatcTAGGTCTTCTTCAGATATCAGTTTCTGTTCCA
p3 TRblue_top NO gat cAG CTG GAA CAG AAA CTG ATC TCT GAA GAA GAC CTA blue oligo record Zra1
p3 TRblue'_btm NO gatcTAGGTCTTCTTCAGAGATCAGTTTCTGTTCCAGCT
p3 TRpurple_top NO gat cTA GAA CAG AAA CTG ATC TCT GAA GAA GAC CTA purple oligo record
p3 TRpurple'_btm NO gatcTAGGTCTTCTTCAGAGATCAGTTTCTGTTCTA
p3 TRred_top NO gat cTA GAA CAG AAA CTG ATC TCT GAA GAA GAC CTA red oligo record XbaI and HindIII
p3 TRred'_btm NO gatcTAGGTCTTCTTCAGAGATCAGTTTCTGTTCTA
p3 TRgreen_top NO gat cTG GAA CAG AAA CTG ATC TCT GAA GAA GAC CTG CT green oligo record BbsI
p3 TRgreen'_btm NO gatcAGCAGGTCTTCTTCAGAGATCAGTTTCTGTTCCA

W/F lab

protein to modify oligo name oligo number oligo sequence primer record enzyme for verification of insert
p3 WFpink_top NO gat c aa GAA CAG AAA CTG ATa TCT GAA GAA GAC CTa pink primer record
p3 WFpink_btm NO gatctAGGGTCTTCTTCAGAtATCAGTTTCTGTTCtt
p3 WFyellow_top NO gat cTA GAA CAG AAG CTT ATC TCT GAA GAA GAC CTC yellow primer record
p3 WFyellow_btm NO g atc GAG GTC TTC TTC AGA GAT AAG CTT CTG TTC TA
p3 WFblue_top NO gat cTC GAA CAG AAA CTG ATA TCT GAA GAA GAC CTT blue primer record EcoRV
p3 WFblue_btm NO gatcAAGGTCTTCTTCAGATATCAGTTTCTGTTCGA and pdf version EcoRV
p8 WFpurple_top NO TCT GAA CAG AAA CTG ATC TCT GAA GAA GAC CTG GGt gca purple primer record Bbs1
p8 WFpurple_btm NO CCCAGGTCTTCTTCAGAGATCAGTTTCTGTTCAGAtgca
p8 WFred_top NO CTG GAA CAG AAA CTG ATC TCT GAA GAA GAC CTG ggt_gca red primer record BbsI
p8 WFred_btm NO ccCAG GTC TTC TTC AGA GAT CAG TTT CTG TTC GAC tgca
p8 WFgreen_top NO TCT GAA CAG AAA CTG ATA TCT GAA GAA GAC CTG GAt gca green primer record EcoRV
p8 WFgreen_btm NO CTCAGGTCTTCTTCAGATATCAGTTTCTGTTCAGAtgca
Personal tools