Talk:20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3)

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Agarose Gels

TR lab

Well folks, today we learned what happens when you switch from 10X to 50X concentrate of TAE buffer, and forget that you have done so! For posterity's sake, your gel images (with their fragments mostly run off) are below. For the For Next Time assignment, please do your calculations using the images from the Wednesday/Friday section.

Agarose gels for Tuesday/Thursday section

Looks like those far-run bands are indeed what we were after. The purified products look good!

Gel with purified samples, T/R section
Lane Sample Lane Sample
1 Ladder 8 Yellow bkb
2 David bkb 9 Yellow frag
3 David frag 10 Green bkb
4 Purple bkb 11 Green frag
5 Purple frag 12 Red/Pink bkb
6 Blue bkb 13 Red/Pink frag
7 Blue frag


The ladder amount loaded results in 125 ng of DNA for the 3 Kbp fragment. Further details about the ladder are here.

David's samples contain 3 μL of DNA. Approximately 20 μL per lane of your samples were loaded (which is how many μL of DNA?).

WF lab

All right, plenty of DNA today! If your ladder bands are difficult to see, go ahead and use someone else's picture to do the calculations for problem 1 of the For Next Time assignment.

Agarose gels for Wednesday/Friday section


Reference gel with pCX-NNX

For some reason the uncut backbone didn't show up too well today (presumably it was at a lower concentration than we thought), so for your your reference I've included an old gel I ran with pCX-NNX above. Uncut plasmid is in lanes 2 and 3.


Gel with purified samples, W/F section
Lane Sample Lane Sample
1 Ladder 8 Green bkb
2 Red bkb 9 Green frag
3 Red frag 10 Blue bkb
4 Pink bkb 11 Blue frag
5 Pink frag (no band) 12 Purple bkb
6 Yellow bkb 13 Purple frag (faint)
7 Yellow frag


The ladder amount loaded results in 125 ng of DNA for the 3 Kbp fragment. Further details about the ladder are here. Approximately 22 μL per lane of your samples were loaded (which is how many μL of DNA?).

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