Talk:20.109(S10):Purify RNA and run affinity column (Day4)

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Sign-up for column protocols

T/R

You and your partner should choose one of the wash protocols below by signing up under the Group heading.

If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.


Protocol# Group 8-12 % Wash # (partner 1) Wash # (partner 2) Note changes to plan here
1 Orange! 2 4 24
2 YELLOW >:] 2 8 16
3 go GREEN go! 10 4 24
4 PINK! 10 8 16
5 PURPLE! <3 50 4 24 We had low concentrations, so we switched with Pink group (who used to be doing this one, and is now doing the washes for which we had originally signed. There was not sufficient 8-12 however to save 1.4 nmol. ;(
6 BLUE 50 8 16
7 Red 50 4 24 We too, had small concentration of 6-5, so we had to not take a sample of it alone. We were also supposed to use a mixture of 10%8-12, but that meant 90% 6-5, and thus for the same reason, we switched to 50:50


W/F

You and your partner should choose one of the wash protocols below by signing up under the Group heading.

If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.


Protocol# Group 8-12 % Wash # (partner 1) Wash # (partner 2) Note changes to plan here
1 Team Purple 2 4 24
2 Yellow :D 2 8 16
3 Orange Team 10 4 24 Since we didn't have enough 6-5 aptamer we ended up making a 50% solution with 4 and 24 washes. Also - our concentrations of RNA were about half of what was expected - but the amount of beads was also half of what the protocol called for so the two should balance out. Agi notes: no benchmark samples either.
4 Team Awesome, i.e. RED EMTs 10 8 16
5 Pink 50 4 24 We switched to a 10% wash because the orange team switched to a 50% wash.
6 Team Blue 50 8 16
7 Green Team 10 8 16


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