Talk:20.109(S11):Prepare DNA for cloning (Day3)

From OpenWetWare

Jump to: navigation, search

Contents

T/R

Record of purification gel

T/R Red, Orange, Yellow Groups
T/R Red, Orange, Yellow Groups
T/R Green, Blue, Pink, Purple Groups
T/R Green, Blue, Pink, Purple Groups



Control digest gel

If you digested for longer than 1 hr or otherwise changed the protocol, please add that to the "Notes" section in the table below.

Control digest gel; samples described in table at right.
Control digest gel; samples described in table at right.
Lane # Group Sample Notes
1 Red XmaI
2 Orange BamHI
3 Orange BamHI
4 Yellow XmaI
5 N/A Undigested 2.5 μL DNA
6 N/A 10 μL Ladder 1 Kbp size from NEB
7 Green BamHI
8 Blue XmaI
9 Pink BamHI
10 Purple XmaI


W/F

Record of purification gel

W/F Red, Orange, Yellow Groups
W/F Red, Orange, Yellow Groups
W/F Green, Blue, Pink, Purple Groups
W/F Green, Blue, Pink, Purple Groups


Control digest gel

If you digested for longer than 1 hr or otherwise changed the protocol, please add that to the "Notes" section in the table below.

Control digest gel; samples described in table at right.
Control digest gel; samples described in table at right.
Lane # Group Sample Notes
1 N/A 10 μL Ladder 1 Kbp size from NEB
2 Red XmaI
3 Orange BamHI
4 Yellow XmaI
5 N/A Undigested 2.5 μL DNA
6 Green BamHI
7 Blue XmaI
8 Pink BamHI
9 Purple XmaI
10 N/A 10 μL Ladder 1 Kbp size from NEB


Personal tools