Talk:Dahlquist:Yeast Transcription Factors

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Kevin, let's use this page to communicate a To Do list. — Kam D. Dahlquist 18:21, 22 September 2008 (EDT)

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Contents

To Do

Wild type

  • Run MAPPFinder for all timepoints, increased and decreased
    • Edit GO results lists for redundancy and group related GO terms
  • STEM clustering (might be done; Kam needs to check)
  • EDGE statistics
  • Re-run clusters/groups of genes through TF finding databases

cin5Δ

  • Run MAPPFinder for all timepoints, increased and decreased
    • Edit GO results lists for redundancy and group related GO terms
  • STEM clustering (might be done; Kam needs to check)
  • EDGE statistics
  • Re-run clusters/groups of genes through TF finding databases

MAPPs

See also MAPP Archive page.

  • One humongous consolidated MAPP (also Excel spreadsheet), then individual MAPPs for each data source. DONE
  • Need to finish Cin5p target MAPPs for: DONE
    • Workman et al.
    • Hu et al.
    • TVSM
    • Yeastract

Comparator Expression Datasets

  • We need to download data from comparator studies, load it into GenMAPP, so that we can analyze them side-by-side with our study.
  • List of comparator datasets has been moved to a new Yeast Cold Shock page

Bibliography

Note to Kevin: if you enclose each reference within its own biblio tags, then your hidden text will reappear, see example below. Kam D. Dahlquist 18:39, 23 December 2008 (EST)

Cin5 Literature Curation Summary from SGD

Error fetching PMID 18156292:
  1. Error fetching PMID 18156292: [Paper1]
  • Overexpression of CIN5 confers resistance to sampagine (involved in oxidative stress)
Error fetching PMID 18287073:
  1. Error fetching PMID 18287073: [Paper2]
  • Explains how CIN5 is related to other YAP family transcription factors. CIN5 involved in DNA damage response and bound to greatest number of genes in DNA damaging conditions. Along with YAP1, CIN5 is a major regulator of the Yap transcriptional network. Provides CIN5 DNA binding motif.
Error fetching PMID 16969341:
  1. Error fetching PMID 16969341: [Paper3]
  • CIN5 involved in downregulation of genes under sudden glucose limitation
Error fetching PMID 16582437:
  1. Error fetching PMID 16582437: [Paper4]
  • A tripple mutant, ΔPAC10ΔPLP1ΔCIN5, shows significantly decreased levels of tubulin (~20% of wildtype). The authors were unsure what role CIN5 plays in the tubulin pathway.
Error fetching PMID 16709784:
  1. Error fetching PMID 16709784: [Paper5]
  • Same paper used in adding to CIN5 TF targets, found under DNA damaging conditions. Shows CIN5 involved in the DNA damage response pathway.
Error fetching PMID 15942868:
  1. Error fetching PMID 15942868: [Paper6]
  • Cin5p interacts with Rns1p, a protein proposed to be inovlved in intracellular protein transport.
Error fetching PMID 15821130:
  1. Error fetching PMID 15821130: [Paper7]
  • CIN5 is involved in the response to chitosan, an antimicrobial drug.
Error fetching PMID 14680476:
  1. Error fetching PMID 14680476: [Paper8]
  • CIN5 is involved in the response to osmotic stress.
Error fetching PMID 15565582:
  1. Error fetching PMID 15565582: [Paper9]
  • CIN5 is involved in the response to heat, oxidative, heavy metal, metalloid stresses. Regulated by phosphorylation.
Error fetching PMID 11410349:
  1. Error fetching PMID 11410349: [Paper10]
  • CIN5 interacts with Knr4p to regulate genes in response to salt stress.
Error fetching PMID 11179441:
  1. Error fetching PMID 11179441: [Paper11]
  • Overexpression of CIN5 confers resistance to cisplatin as well as DNA-alkylating agents methylmethanesulfonate and mitomycin C. Also found that CIN5 is located in the nucleus.
Error fetching PMID 10760578:
  1. Error fetching PMID 10760578: [Paper12]
  • CIN5 interacts with Elc1, which also interacts with other proteins involved in the stress response.
Error fetching PMID 9593122:
  1. Error fetching PMID 9593122: [Paper13]
  • Overexpression of CIN5 confers resistance to quinoline-containing antimalarial drugs quinidine, mefloquine and chloroquine.
Error fetching PMID 9559673:
  1. Error fetching PMID 9559673: [Paper14]
  • Overexpression of CIN5 increases sodium and lithium tolerance.
Error fetching PMID 9372930:
  1. Error fetching PMID 9372930: [Paper15]
  • Explains Yap family of transcription factors. Also mutations in CIN5 cause chromosome instability and suppress cold sensitivity of yap1 mutants.

</biblio>

Short explanation of the relevance of each paper to CIN5 can be seen if you click on "Edit."

MIAME Compliance

  • We need to fill out the "Minimal Information about a Microarray Experiment" information so that when we publish, we can upload the data to a database.
  • MAGE-TAB is one way to do it, but I'm having trouble actually finding a downloadable form that we can fill out. I've e-mailed the MGED "Contact Us" e-mail address asking about it. I guess we can download the paper from that page and try to figure it out ourselves. Kam D. Dahlquist 16:53, 29 October 2008 (EDT)

Completed

Wild type

  • Excel-based statistics
  • multtest

cin5Δ

  • Excel-based statistics
  • multtest

MAPPs

  • consolidated Cin5p target MAPP for Lee, Harbison, Tan. (redundant, gene appears more than once if in multiple papers)
  • individual Cin5p target MAPPs for all sources, as well as one huge Cin5p target MAPP containing genes from Lee, Harbison, Tan, Workman, Hu, YEASTRACT, TVSM
  • Have downloaded Cin5p targets from the following sources:
    • Lee et al.
    • Harbison et al.
    • Tan et al.
      • One consolidated MAPP with Lee, Harbison, and Tan has been completed.
    • Workman et al.
    • Hu et al.
    • TVSM
    • Yeastract
      • MAPPs for each of the above have been created as well.
  • Total compiled Cin5p target MAPP has also been created including all of the data above, saved on desktop in "Database and Reference TF Search" as Cin5Targets_Total_Compiled.mapp. Four total MAPPs were created because the max number of genes was exceeded.
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