Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)

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Results and Discussion

Reaction temparature:37°C,09/12

Sender culture:1000μL,Receiver culture:1000μL

Fig.1  12/09.E.coli strain,senders:JW1908,BBa_T9002:JW1908,Reaction temparature:37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.1  
12/09.E.coli strain,senders:JW1908,BBa_T9002:JW1908,Reaction temparature:37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.


Sender culture:100μL,Receiver culture:1000μL

Fig.2  E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.2  
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.



  • Fig. 1,Fig. 2
  • Results
    • Green fluorescence intensity didn't increase in the culture containing BBa_K0840010(plac+CinI+LVA).We thought BBa_K084010 didn't work properly or LuxR gene didn't interact with signal molecules synthesized by BBa_K084010.
    • Others responded at the same time(4 hours after induction).Final fluorescence intensity differd.

Reaction temparature:37°C

Sender culture:500μLm,Receiver culture:500μL

Fig.3  E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.3  
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.4  E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.4  
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.


  • Fig. 3
  • Results
    • Response time and final fluorescence intensity showed no significant difference.
  • Discussion
    • We thought AHL concentration was quickly reached the threshold concentration.The fluorescence intensity increased as gfp maturured.
  • Fig. 4
  • Results
    • No significant difference in fluorescence intensity between the culture containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.
  • Discussion
    • We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Reaction temparature:30°C

Sender culture:500μL,Receiver culture:500μL

Fig.5  E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.5  
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.6  E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.6  
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.


  • Fig. 6
  • Results
    • No significant difference in fluorescence intensity between the cultures containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.
  • Discussion
    • We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Sender culture:100μL,Receiver culture:1000μL

Fig.7  E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.7  
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.8 E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.8 
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.


  • Fig.7
  • Results
    • No significant difference in fluorescence intensity between the culture containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.
  • Discussion
    • We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Sender culture:10μL,Receiver culture:1000μL

Fig.9  E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Fig.9  
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.


  • Fig.9
  • Results
    • The final fluorescence intensity of the culture containing BBa_K084008 transformed cells which express LVA-tagged RhlI protein was lower than the culture containing cells which express untagged RhlI protein.It shows that LVA-tagged RhlI protein was degraded by LVA-specific protase.However,fluorescence intensity of both cultures increased at the same time.
  • Discussion
    • We thought it was because the AHL concentration was quickly reached the threshold concentration and cells began to express gfp.After 2 hours,fluorescence intensity started to increase.


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