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Welcome to your project page!
For each part listed, you should:
- Design oligos to make your part
- Write up proper construction files and put it on the Construction Files page
- Put your oligos on the Oligo Log page
- Put your part sequence on the part document
- Name your parts according to the list here
EspP Autotransporter
Source: E. coli 0157:H7 genomic DNA
Start by reading PMID: 16262782. You will make a part that begins with amino acid 852 and extends to the stop codon. The DNA sequence for espP is available at: NC_002128.
This part encodes a N-terminal display protein
Your displayer part should be of the {<part!} style (no start, with stop). There should be NO prepro sequence in your part. You should design your construction file to insert your part into plasmid pBca9495KC-Bca1144#5 using EcoRI and BamHI. The map of this plasmid is here.
Myc Tag
Source: pBca1256-K112502 {<myc>}
This part has already been constructed as a BglBricks basic part in plasmid pBca1256. You will be transferring the part using the Gateway reaction into the assembly vector. To do this, you will react the source plasmid with plasmid pBca1254AK. As background, you should read PMID: 12536251.
This part encodes a passenger protein
After performing the Gateway reaction, your product plasmid will be pBca9495AK-partname. To see what the part looks like in the product vector, look at this map of the RFP-expressing part Bca1144 in pBca9495AK.
