Template:SBB10 ConstructionFiles BenB sbb05
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Construction of sbb05: piggieBac 5'TR Pool BDBpb001 through BDBpb010, assemble by PCA PCR BDBpb001/BDBpb010 on PCA reaction (344 bp, EcoRI/BamHI) Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb05 ------------ BDBpb001 PCA assembly of sbb11 CCATAGAATTCATGAGATCTTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGC BDBpb002 PCA assembly of sbb11 GCTATTTAGAAAGAGAGAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATC BDBpb003 PCA assembly of sbb11 GAAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTGTGCATTTAGG BDBpb004 PCA assembly of sbb11 CTCACGGGAGCTCCAAGCGGCGACTGAGATGTCCTAAATGCACAGCGACGGA BDBpb005 PCA assembly of sbb11 CGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCACTGATTTTGA BDBpb006 PCA assembly of sbb11 CGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAGTGACACTTACCGC BDBpb007 PCA assembly of sbb11 CCGCGTGAGTCAAAATGACGCATGATTATCTTTTACGTGACTTTTAAGATTTAACTCATACG BDBpb008 PCA assembly of sbb11 TATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGT BDBpb009 PCA assembly of sbb11 ATATTGTTATTTCATGTTCTACTTACGTGATAACTTATTATATATATATTTTCTTGTTATAGATATCGGATC BDBpb010 PCA assembly of sbb11 CTGATGGATCCGATATCTATAACAAGAAAATATATATATAATAA
JCA Notes
- Correct
