Template:SBB10 ConstructionFiles JennaK sbb29

Biobricking of 099 rep protein basic part sbb29

PCR repo99-F and repo99-R on pEC52 gene             (978 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144                           (EcoRI/BamHI, 918+2472, L)
Product is pBca9523-repo99			     {rbs.repo99!} 
----------------------------
repo99-F   Cloning of repo99   ccaaaGAATTCtaaAGATCTcctggaaggaatcgtaacac
repo99-R   Cloning of repo99   gctagGGATCCctactctacaagacctcg

JCA Notes

• Prefix should be ATG not TAA
• Vector size slightly off
• Reverse oligo can be shared with other part
• Plasmid name should be partName, not partDescription

 Biobricking of 099 rep protein basic part sbb29

 PCR repo99-F and JG002-R on pEC52 gene             (978 bp, EcoRI/BamHI)
 Sub into pBca9523-Bca1144                           (EcoRI/BamHI, 910+2472, L)
 Product is pBca9523-sbb29	{rbs.repO99!} 
 ----------------------------
repo99-F   Cloning of repo99   
ccaaaGAATTCatgAGATCTcctggaaggaatcgtaacac
JG002-R   Cloning of  O99 rep Gene   
CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc