Turner:Solutions/GAMBITMnRxn

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GAMBIT 2x Reaction Buffer + Mn - 10 mL

Component Final [] Stock [] Units mL stock ' '
Glycerol2050%4
HEPES pH 7.90.051M0.5
DTT0.0040.1M0.4
NaCl0.25M0.4
MnCl20.011M0.1
pH to7.9with_(approx)_mL
sterilize byf.s.
store at4C
Note: use 100x BSA from NEB as 40x stock for GAMBIT reaction
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