Turner:Solutions/GAMBITMnRxn

GAMBIT 2x Reaction Buffer + Mn - 10 mL

Component	Final []	Stock []	Units	mL stock	'	'
Glycerol	20	50	%	4
HEPES pH 7.9	0.05	1	M	0.5
DTT	0.004	0.1	M	0.4
NaCl	0.2	5	M	0.4
MnCl2	0.01	1	M	0.1

pH to	7.9	with	_	(approx)	_	mL
sterilize by	f.s.
store at	4C

Note: use 100x BSA from NEB as 40x stock for GAMBIT reaction