UA Biophysics:Protocols

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Contents

ANTIBIOTICS STOCK

Ampicillin
Kanamycin
Chloramphenicol


BACTERIA STOCK

Stocks

BUFFERS

PBS
TAE 10X

CHEMICALS

KOH

EXTRACTION

Lipid Extration
Carotenoid Extration

FLUORESCENCE MICROSCOPY

Tubulin Polymerization with AlexaFluor 488 staining

IMMUNOSTAINING

Lymphocytes immunostaining with anti-AlphaTubulin


MEDIA

M9 Salts (5x) 1L
M9 (5x) 1L
LB 1L
LB Plates 1L
SD 1L
1M MgSO4
0.1M CaCl2

MICROFLUIDICS

Soft lithography
Master fabrication
Plasma Cleaner Protocol
Cell Insertion Mother Machine
Microfluidics Valves
Microfluidic System for Biofilm growth

MOLECULAR BIOLOGY

Q5 Polymerase

Phusion Polymerase

PLASMIDS AND RELATED

GenEluteTM HP Plasmid Miniprep Kit
Preparing Chemically Competent Cells
Transforming Chemically Competent
Competent Cells
Transformation of Competent Cells
Yeast Miniprep

PHAGES

Marking T4 DNA with DAPI

Direct Plating Plaque Assay

Small Drop Plaque Assay System

PREPARACIÓN DE LUV Y GUV

LUV

GUV

TURBIDOSDOSTATS

Plate turbidostat user manual
Revolver turbidostat with interchanging media


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