User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/26

From OpenWetWare

Jump to: navigation, search
Biomaterials Design Lab: Spring 2014 Main project page
Previous entry      Next entry

Objective

  • Record results of 30:1 lysozyme:AuNP (with variables added after baking) that were heated at 37°C overnight.
  • Take UV-Vis of samples with salt added after baking heated at 37°C overnight.
  • Synthesize 30:1 lysozyme-AuNP solutions.
  • Synthesize 60:1, 70:1, 80:1, 90:1, and 100:1 BSA-AuNPs baked with MgCl2 for 4 hours at 80°C.

Procedure

Synthesizing Lysozyme-AuNP and BSA-AuNP

  • The following figure details how the stock solutions were made as well as the BSA-AuNPs and lysozyme-AuNPs.
  • One tube of each ratio was made for BSA-AuNPs.
  • For lysozyme-AuNPs, 25 tubes were made.
  • The samples were heated in the oven at 80°C for 4 hours.

Figures

  • Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. Also the Control Used is the Control at Room Temperature, not at 36 Degrees.

UV-Vis of Heated Samples and Variables (36°C)

Image:Screen_shot_2014-02-26_at_5.18.42_PM.png Image:Screen_shot_2014-02-26_at_5.18.29_PM.png Image:Screen_shot_2014-02-26_at_5.18.18_PM.png Image:Screen_shot_2014-02-26_at_5.17.57_PM.png Image:Screen_shot_2014-02-26_at_5.17.46_PM.png Image:Screen_shot_2014-02-26_at_5.17.36_PM.png Image:Screen_shot_2014-02-26_at_5.17.23_PM.png

  • Based off of all UV-Vis data: The ABS for the controls were corrected for the dilution factor present and the percent AuNP's in solution was calculated.

Image:Screen_Shot_2014-03-26_at_2.53.43_PM.png

Notes

  • These results detail the same samples as seen below; however, these samples were at 37°C before observing.
  • After observations were taken, the samples were placed in the fridge.



  • These results detail the samples from last Thursday with the addition of the variables to 1 mL aliquots of 30:1 ratio with salts added after. The samples were at room temperature (37°C) when observations were taken.


Personal tools