User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/03/04

Biomaterials Design Lab: Spring 2014

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Objective Add the following variables to the 30:1 lysozyme-AuNPs synthesized without salts: NaCl, KCl, MgCl2, CaCl2, 2,2 bipyridine, MES, citric acid. Run UV-Vis of 30:1 lysozyme:AuNP (with variables added after baking) that were cooled to 4°C over the weekend. Procedure Preparing Samples for Atomic Absorption To the 30:1 lysozyme-AuNP samples synthesized with salts, 2.71 mL of the variables were added. Three concentrations for each were tested: 100 mM, 10 mM, and 1 mM. For 2,2 bipyridine, the concentrations were 9 mM, 0.9 mM, and 0.09 mM. The samples were left to sit at room temperature overnight. Figures Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. Also the Control Used is the Control at Room Temperature, not at 4 Degrees. Based off of all UV-Vis data: The ABS for the controls were corrected for the dilution factor present and the percent AuNP's in solution was calculated. Notes Observations of 30:1 lysozyme-AuNP Samples Cooled to 4°C With Variables Added After Synthesizing