User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/03/04

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Biomaterials Design Lab: Spring 2014 Main project page
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Objective

  • Add the following variables to the 30:1 lysozyme-AuNPs synthesized without salts: NaCl, KCl, MgCl2, CaCl2, 2,2 bipyridine, MES, citric acid.
  • Run UV-Vis of 30:1 lysozyme:AuNP (with variables added after baking) that were cooled to 4°C over the weekend.

Procedure

Preparing Samples for Atomic Absorption

  • To the 30:1 lysozyme-AuNP samples synthesized with salts, 2.71 mL of the variables were added. Three concentrations for each were tested: 100 mM, 10 mM, and 1 mM.
  • For 2,2 bipyridine, the concentrations were 9 mM, 0.9 mM, and 0.09 mM.
  • The samples were left to sit at room temperature overnight.

Figures

  • Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. Also the Control Used is the Control at Room Temperature, not at 4 Degrees.

Image:Screen_Shot_2014-03-13_at_2.35.34_PM.png Image:Screen_Shot_2014-03-13_at_2.35.49_PM.png Image:Screen_Shot_2014-03-13_at_2.36.04_PM.png Image:Screen_Shot_2014-03-13_at_2.36.19_PM.png Image:Screen_Shot_2014-03-13_at_2.36.33_PM.png Image:Screen_Shot_2014-03-13_at_2.36.47_PM.png Image:Screen_Shot_2014-03-13_at_2.37.07_PM.png

  • Based off of all UV-Vis data: The ABS for the controls were corrected for the dilution factor present and the percent AuNP's in solution was calculated.

Image:Screen_Shot_2014-03-26_at_2.55.49_PM.png

Notes

Observations of 30:1 lysozyme-AuNP Samples Cooled to 4°C With Variables Added After Synthesizing


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