User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/09

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Biomaterials Design Lab: Spring 2014 Main project page
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Objective

  • Take conductivity measurements on 4°C solutions from 3/26/14.
  • Run UV-Vis and AA on solutions at 36°C from 3/26/14.

Procedure

Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C

  • Please note that these conductivity values are not corrected for their dilution factor. The true conductivity of these solutions must be further back-calculated.
  • Note that MES and 2,2 Bipyridine have reduced conductivity values. This is because other variables are ions, thus further increase this measurement. This may overwhelm observed effects.

Atomic Absorption Preparation

Creating the Gold Stock Solutions

  • Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  • Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  • Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  • Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.* Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at 36°C were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

Figures

Atomic Absorption

  • In comparison to yesterday this AA data has a more recognizable trend. Overall it appears that the amount of gold in solution decreases as the concentration of variable increases.

  • 2,2 bipyridine data was just as wonky as yesterday by having an increase in gold followed by a prompt and steep decrease.

UV-Vis Images

  • Please note that very few samples actually had suspended nanoparticles in solution.

Image:Screen_Shot_2014-04-15_at_7.37.48_PM.png Image:Screen_Shot_2014-04-15_at_7.39.02_PM.png Image:Screen_Shot_2014-04-15_at_7.39.52_PM.png Image:Screen_Shot_2014-04-16_at_3.04.23_PM.png

  • Note that highlighted samples are the only samples with enough of a recognizable peak to calculate an equilibrium constant. Some 2,2 bipyridine data is being left out because it showed no variation from the control.


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