User:Alexander S Mikheyev/Notebook/bee methylome/2010/04/18

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Library preparation

' lane 1 2
Reaction mixtureconc.MatureMature (2nd PCR)
template (after bisulfite conversion)??8.51
PCR primer PE 1.0??0.50.5
PCR primer PE 2.0??0.50.5
Phusion DNA polymerase2x12.512.5
MilliQ310.5
total2525
98C30 sec
98C10 sec
65C30 sec
72C30 sec
25 cycle
72C5 min
Gel extraction by QIAGEN Gel extraction kit
followed by MinElute kit

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Image:0087.jpg



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