User:Alexander S Mikheyev/Notebook/bee methylome/2010/04/18

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Library preparation ' lane 1 2 Reaction mixture conc. Mature Mature (2nd PCR) template (after bisulfite conversion) ?? 8.5 1 PCR primer PE 1.0 ?? 0.5 0.5 PCR primer PE 2.0 ?? 0.5 0.5 Phusion DNA polymerase 2x 12.5 12.5 MilliQ 3 10.5 total 25 25 98C 30 sec 98C 10 sec 65C 30 sec 72C 30 sec 25 cycle 72C 5 min Gel extraction by QIAGEN Gel extraction kit followed by MinElute kit