User:Alexander S Mikheyev/Notebook/mutation accumulation in rotifers/2009/12/04

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Test InstaGene Extraction Quality

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Plate Well Genus 12/2/2009 Count ' Reagents Concentration 1X 15X
Adenita 23Nov09B3Adineta30+H204.7571.25
AV 10-06-09well 6Adineta100+10X Buffer10X115
BostonA1Adineta10+dye10X115
BostonA4Adineta50+dNTP10mM0.23
CCA1Adinetasubs 100+primer mix5uM115
CCA3Adinetasubs 100+Taq5U/uM0.050.75
WH1B3Adineta20+DNA variable2
WH1B5Adineta20+TOTAL10120
WH1B6Adineta50+ Cocktail per well = 8.0ul
WH1C4Adineta30+
WH2A6Adineta10+PrimerHIS F1+R1
WH3A5Adineta20+
WH3C2Adineta7PCR 95C3 min
95C10 sec
EXTRACTION60C2 min
5-10 individuals35 cycles
InstaGene Matrix 200ul per sample
56C30 min
99.9C8min
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