User:Alexander S Mikheyev/Notebook/oil flies/2009/12/13

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Contents

PCR of oil fly bacteria

reagents 1x 15x
water6.191.5
5x7.5112.5
1046R0.34.5
917F0.3
2.5 U/ul Platinum HiFi Pol0.34.5
template0.5
total15


DNA 917F DNA 917F
pit91 gutsTCGAGpit91 skinACTCG
pit91 gutsTCGAGpit91 skinACTCG
pit91 gutsTCGAGpit91 skinACTCG
small pit gutsCACACsmall pit skinATACG
small pit gutsCACACsmall pit skinATACG
small pit gutsCACACsmall pit skinATACG
negativeall 917F
  • 94C 3 min (94C 10s; 57C 45s; 72C 1m)x 20 cycles
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