User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/08

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Contents

To do

  • Organize rotifers
  • Check to see whether PCR product from yesterday is a juno2 transposon

PCR

Reagents 1x conc. 3
h2o5.95-17.85
10x110x3
dye110x3
dNTP0.210 mM0.6
Juno2_F0.410 uM1.2
Juno2_R0.410 uM1.2
Taq0.055 U/um0.15
DNA15 ng/ul
TOTAL10Cocktail per vial9
3m 95C (15s 94C; 2m 60C)x30
  • templates
    • 1:100 dilution of yesterday's PCR product
    • P. rapida genomic
    • P. roseola genomic (+ control)

Image:Gid_2009-11-08_15hr_07min.jpg

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