User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/01

Alien genes

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Test Redesigned Primers PCR Reagents Concentration 1X 5X H20 5.75 28.75 10X Buffer 10X 1 5 dye 10X 1 5 dNTP 10mM 0.2 1 Primers 5uM 1 ** Taq 5U/uM 0.05 0.25 *DNA 20ng/ul 1 5 TOTAL 10 45 Cocktail per well = 9.0ul * DNA Adenita vaga ** Primers AV10126 F2+R2 Adenita ricciae AV10094 F2+R2 HIS F1+R1 HIS F1+R2 PCR 95C 3 min 95C 10 sec 60C 2 min 35 cycles ' vaga ricciae vaga ricciae vaga ricciae vaga ricciae ' 1Kb Ladder well 1 well 2 well 3 well 4 well 5 well 6 well 7 well 8 1Kb Ladder AV10126 AV10126 AV10094 AV10094 HISF1+R1 HISF1+R1 HISF1+R2 HISF1+R2 load 2.0ul ladder and 3.0ul sample on 1%gel