User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/05

From OpenWetWare

Jump to: navigation, search
Alien genes Main project page
Previous entry      Next entry

Contents

InstaGene DNA Quality Check 5475 F + 8047 R (Primer 33)

Plate Well Genus 12/2/2009 Count Sample # ' Reagents Concentration 1X 15X
Adenita 23Nov09B3Adineta30+1H204.7571.25
AV 10-06-09well 6Adineta100+210X Buffer10X115
BostonA1Adineta10+3dye10X115
BostonA4Adineta50+4dNTP10mM0.23
CCA1Adinetasubs 100+5primer mix5uM115
CCA3Adinetasubs 100+6Taq5U/uM0.050.75
WH1B3Adineta20+7DNA variable2
WH1B5Adineta20+8TOTAL10120
WH1B6Adineta50+ 9Cocktail per well = 8.0ul
WH1C4Adineta30+10
WH2A6Adineta10+11Primer5475 F + 8047 R
WH3A5Adineta20+12
WH3C2Adineta713PCR 95C3 min
95C10 sec
EXTRACTION60C2 min
5-10 individuals35 cycles
InstaGene Matrix 200ul per sample
56C30 min
99.9C8min
load 2.0ul 1kb ladder and 2.0ul sample 1% gel
Sample #2 Positive Control
Personal tools