User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/10

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Contents

Adineta Amp Primer 31, 32, 34 and AV10094F2+R2 (Primer 35)

Plate Well Genus 12/2/2009 Count Sample Extract ' Reagents Concentration 1X 14X
AV 10-06-09well 6Adineta100+2H204.7566.5
BostonA1Adineta10+310X Buffer10X114
BostonA4Adineta50+4dye10X114
CCA1Adinetasubs 100+5dNTP10mM0.22.8
CCA3Adinetasubs 100+6primer mix5uM114
WH1B3Adineta20+7use 12/7Taq5U/uM0.050.7
WH1B5Adineta20+8use 12/7DNA variable2
WH1B6Adineta50+ 9use 12/7TOTAL10112
WH1C4Adineta30+10Cocktail per well = 8.0ul
WH2A6Adineta10+11
WH3A5Adineta20+12On Gels
WH3C2Adineta713PrimersAV10007#1-12
AV10002#13-24
4220F+7822R#25-36
EXTRACTIONAV10094F2+R2#37-48
InstaGene Matrix 200ul per sample
56C30 minPCR 95C3 min
99.9C8min95C10 sec
60C1 min
35 cycles
12/2/20095-10 individuals12/7/200910-20 individuals
load 2.0ul 2-log ladder and 2.0ul sample 1% gel
Sample #2 Positive Control
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