User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/15

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Contents

Sample 7,8&9 ReAmp with Primers 1-6

Plate Well Genus 12/2/2009 Count Sample Extract ' Reagents Concentration 1X 5X
AV 10-06-09well 6Adineta100+2H204.7523.75
WH1B3Adineta20+7use 12/710X Buffer10X15
WH1B5Adineta20+8use 12/7dye10X15
WH1B6Adineta50+ 9use 12/7dNTP10mM0.21
primer mix5uM15
Taq5U/uM0.050.25
EXTRACTIONDNA variable2
InstaGene Matrix 200ul per sampleTOTAL1040
56C30 minCocktail per well = 8.0ul
99.9C8min
On Gels
12/2/20095-10 individuals12/7/200910-20 individualsPrimersAV10158#1-4
AV10153#5-8
AV10151#9-12
load 2.0ul 2-log ladder and 2.0ul sample 1% gel AV10150#13-16
Sample #2 Positive ControlAV10140#17-20
AV10134#21-24
PCR 95C3 min
95C10 sec
60C2 min
35 cycles
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