User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/02/16

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Contents

Alien Genes LongPCR

Amplification of AV212_A

Test on CCA3

' [CONC] 1X 5X
Water5.326.5
Takara LA Taq5U0.10.5
Takara LABuffer10X15
dNTP2.5uM each1.68
Template0.2ng/ul15
Primer Mix5uM each1
10.0ul45 / 5 = 9.0ul/ RXN
add 1.0 Primer Mix
PCR Cycle
94C 1 min
98C10 sec
68C15 min
30X
72C10 min
Lane Template Contents ' Fosmid MIX
1CCA3AV10007 FWDAV10011 RVSAV212_A1
2CCA3AV10011 FWDAV10016 RVSAV212_A2
3CCA3AV10016 FWDAV10025 RVSAV212_A3
4CCA3AV10025 FWDAV10028 RVSAV212_A4
5AVAV10007 FWDAV10011 RVSAV212_A1
6AVAV10011 FWDAV10016 RVSAV212_A2
7AVAV10016 FWDAV10025 RVSAV212_A3
8AVAV10025 FWDAV10028 RVSAV212_A4

Loaded 2.0ul sample

HSP and HISTONE

Lane Name
1Avhsp-1_1-1
2Avhsp-1_1-2
3Avhsp-1_2-1
4Avhsp-1_2-2
5Avhsp-1_3-1
6Avhsp-1_3-2
7Avhsp-1_4-1
8Avhsp-1_4-2
9Avhsp-1_5-1
10Avhsp-1_5-2
11Avhsp-1_6-1
12Avhsp-1_6-2
13Avhsp-1_7-1
14Avhsp-1_7-2
15Avhsp-3_1-1
16Avhsp-3_1-2
17Avhsp-3_2-1
18Avhsp-3_2-2
19Avhsp-3_3-1
20Avhsp-3_3-2
21Avhsp-4_1-1
22Avhsp-4_1-2
23Avhsp-4_2-1
24Avhsp-4_2-2
25Avhsp-4_3-1
26Avhsp-4_3-2
27Avhsp-4_4-1
28Avhsp-4_4-2
29Avhis-1_1-1
30Avhis-1_1-2
31Avhis-1_2-1
32Avhis-1_2-2
33Avhis-1_3-1
34Avhis-1_3-2
35Avhis-2_1-1
36Avhis-2_1-2
37Avhis-2_2-1
38Avhis-2_2-2
39Avhis-2_3-1
40Avhis-2_3-2
49Avhis-4_4-1
50Avhis-4_4-2
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