HCO LCO
Amplification of HCO LCO
'
|
[CONC]
|
1X
|
20X
|
'
|
Water |
|
6.05 |
121 |
|
Takara R Buffer |
10X |
1 |
20 |
|
MgCl2 |
25uM |
0.6 |
12 |
|
dNTP |
2.5uM each |
0.8 |
16 |
|
Primer Mix LCO/HCO |
5uM each |
0.5 |
10 |
|
Takara R Taq |
5U |
0.05 |
1 |
|
Template |
InstaGene |
1 |
|
|
|
|
|
180ul / 20 |
9ul / RXN
|
|
PCR Cylce
|
'
|
94C |
30 sec
|
45C |
30 sec
|
72C |
1 min
|
|
30X
|
|
Loaded 2.0ul of sample with 100BP Ladder
AMP Lane
|
Template
|
'
|
1 |
Baltimore |
A1
|
2 |
Baltimore |
B1
|
3 |
Baltimore |
C2
|
4 |
Baltimore |
C3
|
5 |
E. Windsor |
A1
|
6 |
E. Windsor |
A3
|
7 |
E. Windsor |
B1
|
8 |
E. Windsor |
B2
|
9 |
E. Windsor |
C2
|
10 |
Harvard |
A2
|
11 |
Harvard |
A3
|
12 |
Ithaca 1 |
A1
|
13 |
Ithaca 1 |
A2
|
14 |
Ithaca 1 |
A3
|
15 |
Ithaca 1 |
A5
|
labeled |
Cape Cod |
A3
|
labeled |
AV |
|
|