User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/02

From OpenWetWare

Jump to: navigation, search
Alien genes Main project page
Previous entry      Next entry

Contents

Alien Gene LongPCR

Amplification of AV212_A, AV_TEL_L_A, AV_240_B

' [CONC] 1X 9 '
Water5.347.7
Takara LA Taq5U0.10.9
Takara LA Buffer10X19
dNTP2.5uM each1.614.4
*Template1ng19
Primer Mix5uM each1
10.0ul819.0ul/RXN
add 1.0 Primer Mix
* AV or CCA3
PCR Cycle
94C 1 min
98C10 sec
68C15 min
30X

Loaded 2.0ul of sample

AMP Lane Template Contents ' Fosmid MIX
1CCA3AV10011 FWDAV10016 RVSAV212_A2
2AVAV10011 FWDAV10016 RVSAV212_A2
3CCA3AV10094 FWDAV10098 RVSAV_TEL_L_A16
4AVAV10094 FWDAV10098 RVSAV_TEL_L_A16
5CCA3AV10041 FWDAV10042 RVSAV_240_B17
6AVAV10041 FWDAV10042 RVSAV_240_B17
7CCA3AV10041 FWDAV10043 RVSAV_240_B18
8AVAV10041 FWDAV10043 RVSAV_240_B18
9CCA3AV10041 FWDAV10044 RVSAV_240_B19
10AVAV10041 FWDAV10044 RVSAV_240_B19
11CCA3AV10042 FWDAV10045 RVSAV_240_B20
12AVAV10042 FWDAV10045 RVSAV_240_B20
13CCA3AV10043 FWDAV10045 RVSAV_240_B21
14AVAV10043 FWDAV10045 RVSAV_240_B21
15CCA3AV10044 FWDAV10045 RVSAV_240_B22
16AVAV10044 FWDAV10045 RVSAV_240_B22

Alien Genes New Primer Screen

Lane Gene Lane Gene
1AV1015817AV10084
2AV1015318AV10082
3AV1015119AV10075
4AV1015020AV10071
5AV1014021AV10045
6AV1013422AV10043
7AV1013023AV10042
8AV1012324AV10041
9AV1012125AV10034
10AV1010426AV10028
11AV1010927AV10027
12AV1009928AV10025
13AV1009829AV10016
14AV1009430AV10011
15AV1009231AV10007
16AV1008932AV10002
Reaction mixture conc. 1x x33
Temp (Ithaca1 A2,A3,A5)??266
TopTaq buffer10x133
dNTP mix10 mM each0.26.6
Primer mix5 uM each0.5
TopTaq (QIAGEN)5U/ul0.051.65
Milli Q6.25206.25
10 ul
PCR cycle
94C3 min
98C10 sec
60C3 min
x35
72C5 min

Loaded 2.0ul of sample

Image: 2Mar'10_Ithaca_(A2,A3,A5).jpg


Reaction mixture conc. 1x x33
Temp (Ithaca1 A6,B1,B3)??266
TopTaq buffer10x133
dNTP mix10 mM each0.26.6
Primer mix5 uM each1
TopTaq (QIAGEN)5U/ul0.051.65
Milli Q5.75189.75
10 ul
PCR cycle
94C3 min
98C10 sec
60C3 min
x35
72C5 min

Loaded 2.0ul of sample

Image: 2Mar'10_Ithaca_(A6,B1,B3).jpg

Personal tools