User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/11

Alien genes

Main project page Previous entry      Next entry

Alien Genes New Primer Screen & TopTaq VS Takara R Taq Reaction mixture conc. 1x 6 ' Reaction mixture conc. 1x 6 Template CCA3 1ng/ul 1 6 Template CCA3 1ng/ul 1 6 TopTaq buffer 10x 1 Takara R buffer 10x 1 6 dNTP mix 10 mM each 0.2 1.2 MgCl2 25mM 0.6 3.6 Primer mix 5 uM each 0.5 dNTP mix 2.5 mM each 0.8 4.8 TopTaq (QIAGEN) 5U/ul 0.05 0.3 Primer mix 5 uM each 0.5 Milli Q water 7.25 43.5 Takara R Taq 5U/ul 0.05 0.3 10 ul Milli Q water 6.05 36.3 PCR cycle 10 ul 94C 3 min PCR cycle 98C 10 sec 94C 30 sec 60C 3 min 60C 30 sec x35 72C 1 min 72C 5 min x30 Load 5.0ul sample AMP Lane Taq Mix Contents Fosmid 1 Top Taq AV10030 AV240_B 2 Top Taq AV10036 AV240_B 3 Top Taq AV10037 AV240_B 4 Top Taq AV10039 AV240_B 5 Top Taq POS Control AV10008 6 Top Taq no primer 7 Takara R AV10030 AV240_B 8 Takara R AV10036 AV240_B 9 Takara R AV10037 AV240_B 10 Takara R AV10039 AV240_B 11 Takara R POS Control AV10008 12 Takara R no primer