User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/11

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Contents

Alien Genes New Primer Screen & TopTaq VS Takara R Taq

Reaction mixture conc. 1x 6 ' Reaction mixture conc. 1x 6
Template CCA31ng/ul16Template CCA31ng/ul16
TopTaq buffer10x1Takara R buffer10x16
dNTP mix10 mM each0.21.2MgCl225mM0.63.6
Primer mix5 uM each0.5dNTP mix2.5 mM each0.84.8
TopTaq (QIAGEN)5U/ul0.050.3Primer mix5 uM each0.5
Milli Q water7.2543.5Takara R Taq5U/ul0.050.3
10 ulMilli Q water6.0536.3
PCR cycle10 ul
94C3 minPCR cycle
98C10 sec94C30 sec
60C3 min60C30 sec
x3572C1 min
72C5 minx30

Load 5.0ul sample Image:  11march10.tif

AMP Lane Taq Mix Contents Fosmid
1Top TaqAV10030AV240_B
2Top TaqAV10036AV240_B
3Top TaqAV10037AV240_B
4Top TaqAV10039AV240_B
5Top TaqPOS Control AV10008
6Top Taqno primer
7Takara RAV10030AV240_B
8Takara RAV10036AV240_B
9Takara RAV10037AV240_B
10Takara RAV10039AV240_B
11Takara RPOS Control AV10008
12Takara Rno primer
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