Alien Genes New Primer Screen
| Reaction mixture
|
conc.
|
1x
|
x97
|
'
|
No.
|
Primer mix
|
| Temp (49 - 60) |
?? |
2 |
194 |
|
1 |
AV10134
|
| TopTaq Buffer |
10x |
1 |
97 |
|
2 |
AV10121
|
| dNTP mix |
10 mM each |
0.2 |
19.4 |
|
3 |
AV10084
|
| Primer mix |
5 uM each |
1 |
|
|
4 |
AV10045
|
| TopTaq |
5 U/ul |
0.05 |
4.85 |
|
5 |
AV10044
|
| Milli Q |
|
5.75 |
557.75 |
|
6 |
AV10034
|
|
|
10 ul |
|
|
7 |
AV10027
|
|
|
|
|
|
8 |
AV10025
|
| PCR cycle |
|
|
|
|
|
|
| 94C |
3 min |
|
|
|
No. |
|
|
|
|
|
|
27 |
Ithaca 2 A3
|
| 98C |
10 sec |
|
|
|
28 |
Ithaca 2 A4
|
| 60C |
3 min |
|
|
|
29 |
Ithaca 2 A5
|
| x35 |
|
|
|
|
37 |
Tsukuba 3 D2
|
|
|
|
|
|
40 |
Woods Hole 1 A6
|
| 72C |
5 min |
|
|
|
43 |
Yokohama B5
|
|
|
|
|
|
47 |
Harvard A1
|
|
|
|
|
|
48 |
Ithaca 2 A6
|
|
|
|
|
|
49 |
Pennsylvania (PA) D4
|
|
|
|
|
|
50 |
Tsukuba 1 B5
|
|
|
|
|
|
51 |
Yokohama A2
|
|
|
|
|
|
55 |
Yokohama B2
|
loaded 2.0ul of each sample
Apis mellifera methylome
| Sample (Honey bee brain)
|
'
|
Program
|
|
|
| Callow worker |
6.1 ug/100ul |
Target Base Pair (Peak) 300bp
|
| Mature worker |
3.8 ug/100ul |
|
|
|
Duty Cycle 10%
|
|
|
Intensity 4
|
|
|
Cycle per Burst 200
|
|
|
Time (second) 120
|
|
|
Chiller Temperature 19.5C
|
Loaded 5.0ul/100.0ul of each sample
| 
Alien genes
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