User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/30

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Alien Genes New Primer Screen

Reaction mixture conc. 1x x97 ' No. Primer mix
Temp (49 - 60)??21941AV10134
TopTaq Buffer10x1972AV10121
dNTP mix10 mM each0.219.43AV10084
Primer mix5 uM each14AV10045
TopTaq5 U/ul0.054.855AV10044
Milli Q5.75557.756AV10034
10 ul7AV10027
8AV10025
PCR cycle
94C3 minNo.
27Ithaca 2 A3
98C10 sec28Ithaca 2 A4
60C3 min29Ithaca 2 A5
x3537Tsukuba 3 D2
40Woods Hole 1 A6
72C5 min43Yokohama B5
47Harvard A1
48Ithaca 2 A6
49Pennsylvania (PA) D4
50Tsukuba 1 B5
51Yokohama A2
55Yokohama B2


loaded 2.0ul of each sample

image:0056.jpg


Apis mellifera methylome

Sample (Honey bee brain) ' Program
Callow worker6.1 ug/100ulTarget Base Pair (Peak) 300bp
Mature worker3.8 ug/100ul
Duty Cycle 10%
Intensity 4
Cycle per Burst 200
Time (second) 120
Chiller Temperature 19.5C

Loaded 5.0ul/100.0ul of each sample

image:0054.jpg


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