User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/21

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Contents

Nuclear Gene HS Takara PCR Optimization

Mix A : HS Takara RXN Mixture conc 1X 6.5 '
Milli Q water3.120.15
Takara HS Buffer10X213
dNTP2.5uM0.85.2
Primer Mix20uM4
HS Taq5U0.10.65
TemplateInstagene213
528.0ul / RXN
Mix B : HS Takara RXN Mixtureconc1X6.5
Milli Q water3.120.15
Takara HS Buffer10X213
dNTP2.5uM0.85.2
Primer Mix20uM3
HS Taq5U0.10.65
TemplateInstagene213
527.0ul / RXN
Mix C : HS Takara RXN Mixtureconc1X6.5
Milli Q water3.120.15
Takara HS Buffer10X213
dNTP2.5uM0.85.2
Primer Mix20uM4
HS Taq5U0.10.65
TemplateInstagene213
526.0ul / RXN
A PCR cycle
95C3 min
95C5 sec
60C1 min
x35
*AV 1ng Positive Control
AMP Lane Template 2.0ul Primer AMP Lane Template 3.0ul Primer AMP Lane Template 4.0ul Primer
171 InstageneA11971 InstageneA13771 InstageneA1
271 InstageneA22071 InstageneA23871 InstageneA2
371 InstageneA32171 InstageneA33971 InstageneA3
471 InstageneA42271 InstageneA44071 InstageneA4
571 InstageneA52371 InstageneA54171 InstageneA5
671 InstageneA62471 InstageneA64271 InstageneA6
772 InstageneA12572 InstageneA14372 InstageneA1
872 InstageneA22672 InstageneA24472 InstageneA2
972 InstageneA32772 InstageneA34572 InstageneA3
1072 InstageneA42872 InstageneA44672 InstageneA4
1172 InstageneA52972 InstageneA54772 InstageneA5
1272 InstageneA63072 InstageneA64872 InstageneA6
13AV 1ngA131AV 1ngA149AV 1ngA1
14AV 1ngA232AV 1ngA250AV 1ngA2
15AV 1ngA333AV 1ngA351AV 1ngA3
16AV 1ngA434AV 1ngA452AV 1ngA4
17AV 1ngA535AV 1ngA553AV 1ngA5
18AV 1ngA636AV 1ngA654AV 1ngA6

Loaded 2.0ul Sample

Image:21April10A.jpg

Reran Control PCR product from above - sample PCR Program

Loaded 2.0ul Sample

Image:21April10B.jpg

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