User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/23

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Contents

Nuclear Gene HS Takara PCR Optimization

HS Takara RXN Mixture conc 1X 6.5 '
Milli Q water4.126.65
Takara HS Buffer10X213
dNTP2.5uM0.85.2
Primer Mix20uM2
HS Taq5U0.10.65
TemplateInstagene / 1ng213
58.59.0ul / RXN
A PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplate2.0ul Primer
1AV InstageneA1
2AV InstageneA2
3AV InstageneA3
4AV InstageneA4
5AV InstageneA5
6AV InstageneA6
7AV 1ngA1
8AV 1ngA2
9AV 1ngA3
10AV 1ngA4
11AV 1ngA5
12AV 1ngA6


Load 2.0ul Sample

Image:23April10A.jpg

Nuclear Gene EX HS Takara PCR Optimization

EX Takara RXN Mixture conc 1X 6.5 '
Milli Q water5.1533.475
Takara EX HS Buffer10X16.5
dNTP2.5uM0.85.2
Primer Mix20uM1
EX HS Taq5U0.050.325
TemplateInstagene213
58.59.0ul / RXN
A PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplate2.0ul Primer
171 InstageneA1
271 InstageneA2
371 InstageneA3
471 InstageneA4
571 InstageneA5
671 InstageneA6
772 InstageneA1
872 InstageneA2
972 InstageneA3
1072 InstageneA4
1172 InstageneA5
1272 InstageneA6
13AV InstageneA1
14AV InstageneA2
15AV InstageneA3
16AV InstageneA4
17AV InstageneA5
18AV InstageneA6
19AV 1ngA1
20AV 1ngA2
21AV 1ngA3
22AV 1ngA4
23AV 1ngA5
24AV 1ngA6



Load 2.0ul Sample

Image:23April10B.jpg

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