User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/12

Alien genes

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Nuclear Gene 454 Adenita Amplification Re-Amp Cultures 7-10 EX Takara RXN Mixture conc 1X 5 Milli Q water 4.15 20.75 Takara EX Buffer 10X 1 5 dNTP 2.5uM 0.8 4 Primer Mix 20uM 1 EX Taq 5U 0.05 0.25 Template Instagene 3 15 45 9.0ul / RXN add 1.0ul Primer PCR cycle 98C 10 sec 60C 1 min x35 AMP Lane Template Primer 1 7 Instagene B7 2 7 Instagene C7 3 7 Instagene D7 4 7 Instagene E7 5 7 Instagene F7 6 8 Instagene B8 7 8 Instagene C8 8 8 Instagene D8 9 8 Instagene E8 10 8 Instagene F8 11 9 Instagene B9 12 9 Instagene C9 13 9 Instagene D9 14 9 Instagene E9 15 9 Instagene F9 16 10 Instagene B10 17 10 Instagene C10 18 10 Instagene D10 19 10 Instagene E10 20 10 Instagene F10 21 Neg Control B7 22 Neg Control C7 23 Neg Control D7 24 Neg Control E7 25 Neg Control F7 26 Pos Control B7 27 Pos Control C7 28 Pos Control D7 29 Pos Control E7 30 Pos Control F7 *Positive Control = AV 20ng Load 2.0ul Sample Nuclear Gene 454 Adenita Amplification Re-Amp Cultures 11-12 Amp Cultures 13-16 EX Takara RXN Mixture conc 1X 5 Milli Q water 4.15 20.75 Takara EX Buffer 10X 1 5 dNTP 2.5uM 0.8 4 Primer Mix 20uM 1 EX Taq 5U 0.05 0.25 Template Instagene 3 15 45 9.0ul / RXN add 1.0ul Primer PCR cycle 98C 10 sec 60C 1 min x35 AMP Lane Template Primer 1 11 Instagene B11 2 11 Instagene C11 3 11 Instagene D11 4 11 Instagene E11 5 11 Instagene F11 6 12 Instagene B12 7 12 Instagene C12 8 12 Instagene D12 9 12 Instagene E12 10 12 Instagene F12 11 13 Instagene B1 12 13 Instagene C1 13 13 Instagene D1 14 13 Instagene E1 15 13 Instagene F1 16 14 Instagene B2 17 14 Instagene C2 18 14 Instagene D2 19 14 Instagene E2 20 14 Instagene F2 21 15 Instagene B3 22 15 Instagene C3 23 15 Instagene D3 24 15 Instagene E3 25 15 Instagene F3 26 16 Instagene B4 27 16 Instagene C4 28 16 Instagene D4 29 16 Instagene E4 30 16 Instagene F4 31 Neg Control B1 32 Neg Control C1 33 Neg Control D1 34 Neg Control E1 35 Neg Control F1 36 Pos Control B1 37 Pos Control C1 38 Pos Control D1 39 Pos Control E1 40 Pos Control F1 *Positive Control = AV 20ng Load 2.0ul Sample Nuclear Gene 454 Adenita Amplification Amp Cultures 17-24 EX Takara RXN Mixture conc 1X 5 ' ' Milli Q water 4.15 20.75 Takara EX Buffer 10X 1 5 dNTP 2.5uM 0.8 4 Primer Mix 20uM 1 EX Taq 5U 0.05 0.25 Template Instagene 3 15 45 9.0ul / RXN add 1.0ul Primer PCR cycle 98C 10 sec 60C 1 min x35 AMP Lane Template Primer AMP Lane Template Primer 1 17 Instagene B5 26 22 Instagene B10 2 17 Instagene C5 27 22 Instagene C10 3 17 Instagene D5 28 22 Instagene D10 4 17 Instagene E5 29 22 Instagene E10 5 17 Instagene F5 30 22 Instagene F10 6 18 Instagene B6 31 23 Instagene B11 7 18 Instagene C6 32 23 Instagene C11 8 18 Instagene D6 33 23 Instagene D11 9 18 Instagene E6 34 23 Instagene E11 10 18 Instagene F6 35 23 Instagene F11 11 19 Instagene B7 36 24 Instagene B12 12 19 Instagene C7 37 24 Instagene C12 13 19 Instagene D7 38 24 Instagene D12 14 19 Instagene E7 39 24 Instagene E12 15 19 Instagene F7 40 24 Instagene F12 16 20 Instagene B8 41 Neg Control B5 17 20 Instagene C8 42 Neg Control C5 18 20 Instagene D8 43 Neg Control D5 19 20 Instagene E8 44 Neg Control E5 20 20 Instagene F8 45 Neg Control F5 21 21 Instagene B9 46 Pos Control B5 22 21 Instagene C9 47 Pos Control C5 23 21 Instagene D9 48 Pos Control D5 24 21 Instagene E9 49 Pos Control E5 25 21 Instagene F9 50 Pos Control F5 *Positive Control = AV 20ng Load 2.0ul Sample