User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/15

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Contents

Nuclear Region Adenita Culture 454 Amplification

Adenita Cultures 41-44

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water5.1525.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene210
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
141 InstageneB51644 InstageneB8
241 InstageneC51744 InstageneC8
341 InstageneD51844 InstageneD8
441 InstageneE51944 InstageneE8
541 InstageneF52044 InstageneF8
642 InstageneB621Neg ControlB5
742 InstageneC622Neg ControlC5
842 InstageneD623Neg ControlD5
942 InstageneE624Neg ControlE5
1042 InstageneF625Neg ControlF5
1143 InstageneB726Pos ControlB5
1243 InstageneC727Pos ControlC5
1343 InstageneD728Pos ControlD5
1443 InstageneE729Pos ControlE5
1543 InstageneF730Pos ControlF5
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:15May10A.jpg

Nuclear Region Adenita Culture 454 Amplification Status

CULTURE PRIMER COLUMN GOOD REDO? REDO Result
Instagene 1B1-F11Y 10May
Instagene 2B2-F21Y 10May
Instagene 3B3-F31Y 11May
Instagene 4B4-F41Y 11May
Instagene 5B5-F51Y 11May
Instagene 6B6-F61Y 11May
Instagene 7B7-F71Y 12May
Instagene 8B8-F81Y 12May
Instagene 9B9-F91Y 12May
Instagene 10B10-F101B10, C10, F10 weak 12May
Instagene 11B11-F111XFailed
Instagene 12B12-F121Y 12 May
Instagene 13B1-F12WEAK 12May
Instagene 14B2-F22Y 12 May
Instagene 15B3-F32Y 12 May
Instagene 16B4-F42Y 12 May
Instagene 17B5-F52D5, E5, F5 weak 12MayB5, C5, D5, E5, F5 13May
Instagene 18B6-F62XFailed
Instagene 19B7-F72D7, E7, F7 weak 12MayD7 13May
Instagene 20B8-F82C8
Instagene 21B9-F92X
Instagene 22B10-F102y 14MayD10, E10 12May
Instagene 23B11-F112y 14MayC11, D11, E11, F11 12May
Instagene 24B12-F122y 14MayX
Instagene 25B1-F13y 14MayD1 13May
Instagene 26B2-F23Y 13May
Instagene 27B3-F33D6 weak 13Mayfailed 14May
Instagene 28B4-F43B7, C7 13Mayonly C4 14May
Instagene 29B5-F53C5 13 MayC5 14May
Instagene 30B6-F63Y 14MayB6, C6, D6 13 May
Instagene 31B7-F73XB7, C7, E7 14May
Instagene 32B8-F83Xfailed
Instagene 33B9-F93X
Instagene 34B10-F103X
Instagene 35B11-F113X
Instagene 36B12-F123X
Instagene 37B1-F14x
Instagene 38B2-F24x
Instagene 39B3-F34x
Instagene 40B4-F44x
Instagene 41B5-F54x
Instagene 42B6-F64x
Instagene 43B7-F74x
Instagene 44B8-F84xD8, E8 15May
Instagene 45B9-F94
Instagene 46B10-F104
Instagene 47B11-F114
Instagene 48B12-F124
Instagene 49B1-F15
Instagene 50B2-F25
Instagene 51B3-F35
Instagene 52B4-F45
Instagene 53B5-F55
Instagene 54B6-F65
Instagene 55B7-F75
Instagene 56B8-F85
Instagene 57B9-F95
Instagene 58B10-F105
Instagene 59B11-F115
Instagene 60B12-F125
Instagene 61B1-F16
Instagene 62B2-F26
Instagene 63B3-F36
Instagene 64B4-F46
Instagene 65B5-F56
Instagene 66B6-F66
Instagene 67B7-F76
Instagene 68B8-F86
Instagene 69B9-F96
Instagene 70B10-F106
Instagene 71B11-F116
Instagene 72B12-F126
Instagene 73B1-F17
Instagene 74B2-F27
Instagene 75B3-F37
Instagene 76B4-F47
Instagene 77B5-F57
Instagene 78B6-F67
Instagene 79B7-F77
Instagene 80B8-F87
Instagene 81B9-F97
Instagene 82B10-F107
Instagene 83B11-F117
Instagene 84B12-F127
Instagene 85B1-F18
Instagene 86B2-F28
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