Nuclear Region Adenita Culture 454 Amplification
Reaction mixture
|
conc.
|
1x
|
x61
|
'
|
lane
|
Template
|
Primer mix
|
Template |
?? |
3 |
183 |
|
1 |
Harvard A2 (#10) |
B10
|
ExTaq buffer |
10x |
1 |
61 |
|
2 |
|
C10
|
dNTP mix |
2.5 mM each |
0.8 |
48.8 |
|
3 |
|
D10
|
Primer mix |
20 uM each |
1 |
|
|
4 |
|
E10
|
ExTaq |
5 U/ul |
0.05 |
3.05 |
|
5 |
|
F10
|
Milli Q |
|
4.15 |
253.15 |
|
6 |
Harvard A3 (#11) |
B11
|
|
|
10 ul |
|
|
7 |
|
C11
|
|
|
|
|
|
8 |
|
D11
|
PCR cycle |
|
|
|
|
9 |
|
E11
|
98C |
10 sec |
|
|
|
10 |
|
F11
|
60C |
1 min |
|
|
|
11 |
Ithaca 1 B1 (#17) |
B5
|
x35 |
|
|
|
|
12 |
|
C5
|
|
|
|
|
|
13 |
|
D5
|
|
|
|
|
|
14 |
|
E5
|
|
|
|
|
|
15 |
|
F5
|
|
|
|
|
|
16 |
Ithaca 1 B3 (#18) |
B6
|
|
|
|
|
|
17 |
|
C6
|
|
|
|
|
|
18 |
|
D6
|
|
|
|
|
|
19 |
|
E6
|
|
|
|
|
|
20 |
|
F6
|
|
|
|
|
|
21 |
Ithaca 1 B5 (#19) |
B7
|
|
|
|
|
|
22 |
|
C7
|
|
|
|
|
|
23 |
|
D7
|
|
|
|
|
|
24 |
|
E7
|
|
|
|
|
|
25 |
|
F7
|
|
|
|
|
|
26 |
Ithaca 1 C1 (#20) |
B8
|
|
|
|
|
|
27 |
|
C8
|
|
|
|
|
|
28 |
|
D8
|
|
|
|
|
|
29 |
|
E8
|
|
|
|
|
|
30 |
|
F8
|
|
|
|
|
|
31 |
Ithaca 1 D1 (#21) |
B9
|
|
|
|
|
|
32 |
|
C9
|
|
|
|
|
|
33 |
|
D9
|
|
|
|
|
|
34 |
|
E9
|
|
|
|
|
|
35 |
|
F9
|
|
|
|
|
|
36 |
Ithaca 2 A3 (#27) |
B3
|
|
|
|
|
|
37 |
|
C3
|
|
|
|
|
|
38 |
|
D3
|
|
|
|
|
|
39 |
|
E3
|
|
|
|
|
|
40 |
|
F3
|
|
|
|
|
|
41 |
Ithaca 2 A4 (#28) |
B4
|
|
|
|
|
|
42 |
|
C4
|
|
|
|
|
|
43 |
|
D4
|
|
|
|
|
|
44 |
|
E4
|
|
|
|
|
|
45 |
|
F4
|
|
|
|
|
|
46 |
Ithaca 2 A5 (#29) |
B5
|
|
|
|
|
|
47 |
|
C5
|
|
|
|
|
|
48 |
|
D5
|
|
|
|
|
|
49 |
|
E5
|
|
|
|
|
|
50 |
|
F5
|
|
|
|
|
|
51 |
MilliQ (negative control) |
B10
|
|
|
|
|
|
52 |
|
C10
|
|
|
|
|
|
53 |
|
D10
|
|
|
|
|
|
54 |
|
E10
|
|
|
|
|
|
55 |
|
F10
|
|
|
|
|
|
56 |
AV_HMW (20ug) |
B10
|
|
|
|
|
|
57 |
|
C10
|
|
|
|
|
|
58 |
|
D10
|
|
|
|
|
|
59 |
|
E10
|
|
|
|
|
|
60 |
|
F10
|
load 2.0 ul of sample
|