User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/06/18

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Contents

Nuclear Region 454 Amplification

Oleg Samples Amplified with primer no adapters

Extend with primers w/adapters


Image:OlegNewExtraction.jpg


EX Takara RXN Mixture conc 1X 8 '
Milli Q water6.1549.2
Takara EX Buffer10X18
dNTP2.5uM0.86.4
Primer Mix20uM1
EX Taq5U0.050.4
TemplateOleg PCR 1:101
64
8.0ul / RXN add 1.0ul Primer and 1.0ul PCR template
PCR cycle
98C10 sec
60C1 min
x5
AMP LaneTemplatePrimer
1Culture 11B11
2Culture 11D11
3Culture 11E11
4Culture 11F11
5Culture HB12
6Culture HC12
7Culture HE12
8Culture HF12


Load 2.0ul of sample

Image:18June10.jpg


Placed same PCR on machine for an additional 15 cycles


Load 2.0ul of sample

Image:18June10B.jpg

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