User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/06/21

Alien genes

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Adenita Culture 454 Amplification Adapter extension EX Takara RXN Mixture conc 1X 16 ' ' Milli Q water 6.15 98.4 Takara EX Buffer 10X 1 16 dNTP 2.5uM 0.8 12.8 Primer Mix 20uM 1 EX Taq 5U 0.05 0.8 Template Oleg PCR 1:10 1 PCR 1:5 128 8.0ul / RXN add 1.0ul Primer and 1.0ul PCR template PCR cycle 98C 10 sec 60C 1 min x15 AMP Lane Template Primer AMP Lane Template Primer 1 Culture 11 1:5 B11 1 Culture 11 1:10 B11 2 Culture 11 1:5 D11 2 Culture 11 1:10 D11 3 Culture 11 1:5 E11 3 Culture 11 1:10 E11 4 Culture 11 1:5 F11 4 Culture 11 1:10 F11 5 Culture H 1:5 B12 5 Culture H 1:10 B12 6 Culture H 1:5 C12 6 Culture H 1:10 C12 7 Culture H 1:5 E12 7 Culture H 1:10 E12 8 Culture H 1:5 F12 8 Culture H 1:10 F12 Load 2.0ul of sample