AMPure Cleaned 454 Alien Gene Products
1) Combined 5.0ul of each culture #1-12 = 8 tubes
2) Cleaned with AMPure beads http://www.beckmangenomics.com/products/dna_purification_and_cleanup/agencourt_ampure_xp.html
3) Eluted with 40.0ul EB Buffer from Qiagen Extraction Kit.
Loaded 2.0ul on Gel
| CULTURE
|
PRIMER
|
MIX TUBE
|
| 1 |
B1-F1 |
A
|
| 2 |
B2-F2 |
A
|
| 3 |
B3-F3 |
A
|
| 4 |
B4-F4 |
A
|
| 5 |
B5-F5 |
A
|
| 6 |
B6-F6 |
A
|
| 7 |
B7-F7 |
A
|
| 8 |
B8-F8 |
A
|
| 9 |
B9-F9 |
A
|
| 10 |
B10-F10 |
A
|
| 11 |
B11-F11 |
A
|
| 12 |
B12-F12 |
A
|
| 13 |
B1-F1 |
B
|
| 14 |
B2-F2 |
B
|
| 15 |
B3-F3 |
B
|
| 16 |
B4-F4 |
B
|
| 17 |
B5-F5 |
B
|
| 18 |
B6-F6 |
B
|
| 19 |
B7-F7 |
B
|
| 20 |
B8-F8 |
B
|
| 21 |
B9-F9 |
B
|
| 22 |
B10-F10 |
B
|
| 23 |
B11-F11 |
B
|
| 24 |
B12-F12 |
B
|
| 25 |
B1-F1 |
C
|
| 26 |
B2-F2 |
C
|
| 27 |
B3-F3 |
C
|
| 28 |
B4-F4 |
C
|
| 29 |
B5-F5 |
C
|
| 30 |
B6-F6 |
C
|
| 31 |
B7-F7 |
C
|
| 32 |
B8-F8 |
C
|
| 33 |
B9-F9 |
C
|
| 34 |
B10-F10 |
C
|
| 35 |
B11-F11 |
C
|
| 36 |
B12-F12 |
C
|
| 37 |
B1-F1 |
D
|
| 38 |
B2-F2 |
D
|
| 39 |
B3-F3 |
D
|
| 40 |
B4-F4 |
D
|
| 41 |
B5-F5 |
D
|
| 42 |
B6-F6 |
D
|
| 43 |
B7-F7 |
D
|
| 44 |
B8-F8 |
D
|
| 45 |
B9-F9 |
D
|
| 46 |
B10-F10 |
D
|
| 47 |
B11-F11 |
D
|
| 48 |
B12-F12 |
D
|
| 49 |
B1-F1 |
E
|
| 50 |
B2-F2 |
E
|
| 51 |
B3-F3 |
E
|
| 52 |
B4-F4 |
E
|
| 53 |
B5-F5 |
E
|
| 54 |
B6-F6 |
E
|
| 55 |
B7-F7 |
E
|
| 56 |
B8-F8 |
E
|
| 57 |
B9-F9 |
E
|
| 58 |
B10-F10 |
E
|
| 59 |
B11-F11 |
E
|
| 60 |
B12-F12 |
E
|
| 61 |
B1-F1 |
F
|
| 62 |
B2-F2 |
F
|
| 63 |
B3-F3 |
F
|
| 64 |
B4-F4 |
F
|
| 65 |
B5-F5 |
F
|
| 66 |
B6-F6 |
F
|
| 67 |
B7-F7 |
F
|
| 68 |
B8-F8 |
F
|
| 69 |
B9-F9 |
F
|
| 70 |
B10-F10 |
F
|
| 71 |
B11-F11 |
F
|
| 72 |
B12-F12 |
F
|
| 73 |
B1-F1 |
G
|
| 74 |
B2-F2 |
G
|
| 75 |
B3-F3 |
G
|
| 76 |
B4-F4 |
G
|
| A |
B5-F5 |
G
|
| B |
B6-F6 |
G
|
| C |
B7-F7 |
G
|
| D |
B8-F8 |
G
|
| E |
B9-F9 |
G
|
| F |
B10-F10 |
G
|
| G |
B11-F11 |
G
|
| H |
B12-F12 |
G
|
| I |
B1-F1 |
H
|
| CCA3 |
B2-F2 |
H
|
| A. ricciae |
B3-F3 |
H
|
|
|
Yeast Culture Prep for Long Term Freezer Storage
From Revived Yeast Culture
NCYC2722
1) Overnight culture on agar plate (or a few day until lawn is of sufficient size)
Streaked out 50.0ul of growing culture onto a glucose+yeast extract plate
S. pombe - failed to grow - redo
S. cerevisiae - good lawn
2) Prepare freezing media = 20% gycerol in sterile YM media. Aliquot into small freezer tube (1000ul).
3) Collect lawn with sterile toothpick and swirl in freezing media
4) Slow freeze in -30C
|
Alien genes
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