User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/12

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AMPure Cleaned 454 Alien Gene Products

1) Combined 5.0ul of each culture #1-12 = 8 tubes 2) Cleaned with AMPure beads http://www.beckmangenomics.com/products/dna_purification_and_cleanup/agencourt_ampure_xp.html 3) Eluted with 40.0ul EB Buffer from Qiagen Extraction Kit.

Loaded 2.0ul on Gel

Image:12July10.jpg

CULTURE PRIMER MIX TUBE
1B1-F1A
2B2-F2A
3B3-F3A
4B4-F4A
5B5-F5A
6B6-F6A
7B7-F7A
8B8-F8A
9B9-F9A
10B10-F10A
11B11-F11A
12B12-F12A
13B1-F1B
14B2-F2B
15B3-F3B
16B4-F4B
17B5-F5B
18B6-F6B
19B7-F7B
20B8-F8B
21B9-F9B
22B10-F10B
23B11-F11B
24B12-F12B
25B1-F1C
26B2-F2C
27B3-F3C
28B4-F4C
29B5-F5C
30B6-F6C
31B7-F7C
32B8-F8C
33B9-F9C
34B10-F10C
35B11-F11C
36B12-F12C
37B1-F1D
38B2-F2D
39B3-F3D
40B4-F4D
41B5-F5D
42B6-F6D
43B7-F7D
44B8-F8D
45B9-F9D
46B10-F10D
47B11-F11D
48B12-F12D
49B1-F1E
50B2-F2E
51B3-F3E
52B4-F4E
53B5-F5E
54B6-F6E
55B7-F7E
56B8-F8E
57B9-F9E
58B10-F10E
59B11-F11E
60B12-F12E
61B1-F1F
62B2-F2F
63B3-F3F
64B4-F4F
65B5-F5F
66B6-F6F
67B7-F7F
68B8-F8F
69B9-F9F
70B10-F10F
71B11-F11F
72B12-F12F
73B1-F1G
74B2-F2G
75B3-F3G
76B4-F4G
AB5-F5G
BB6-F6G
CB7-F7G
DB8-F8G
EB9-F9G
FB10-F10G
GB11-F11G
HB12-F12G
IB1-F1H
CCA3B2-F2H
A. ricciaeB3-F3H

Yeast Culture Prep for Long Term Freezer Storage

From Revived Yeast Culture NCYC2722


1) Overnight culture on agar plate (or a few day until lawn is of sufficient size)

Streaked out 50.0ul of growing culture onto a glucose+yeast extract plate

S. pombe - failed to grow - redo S. cerevisiae - good lawn

2) Prepare freezing media = 20% gycerol in sterile YM media. Aliquot into small freezer tube (1000ul).

3) Collect lawn with sterile toothpick and swirl in freezing media

4) Slow freeze in -30C

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