User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/08/11

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Contents

MATA2 PCR on S. cerevisiae

EX Takara RXN Mixture conc 1X 6
Milli Q water6.1536.9
Takara EX Buffer10X16
dNTP2.5uM0.84.8
Primer Mix5uM16
EX Taq5U0.050.3
TemplateChelex Extraction1
54
9.0ul / RXN and 1.0ul Chelex Extraction
GenusspeciesNCYC #sample
Saccharomycescerevisiae6089318
Saccharomycescerevisiae6089419
Saccharomycescerevisiae6089520
Saccharomycescerevisiae6089621
Saccharomycescerevisiae1001(+)
Negative Control(-)
PCR cycle
98C10 sec
60C1 min
x15

Image:11Aug2010.jpg

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