User:Alicia Rasines Mazo/Notebook/CHEM-572 Experimental Biological Chemistry II/2015/01/28

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Tasks for January 28

  • To determine the size and concentration of Gold/Lysozyme nanoparticles from UV/vis spectra

Determination of size and concentration of Gold/Lys nanoparticles

  • Followed method described by Wolfgang Heiss et al.
  • Measured highest peak absorbance and divided by absorbance at 450 nm to get a ratio that can be compared to tabulated values. Then matched diameter with tabulated molar absorptivity value. Found concentration using the following equation:
    • c=A450450
    • UV/Vis wavelength range: 400-800 nm
Sample SPR Wavelength SPR Abs Abs 450 Abs Water at 450 SPR Corrected Abs Water at 529 A450 Corrected Aspr/A450 Diameter (nm) ε450 Concentration(M) Concentration (nM)
15290,850,5960,0580,7960,0540,5381,4795539039,55,65E+079,52E-099,52
25290,8440,5960,0580,790,0540,5381,4684014879,255,40E+079,96E-099,96
35290,8880,6270,0580,8340,0540,5691,465729359,255,40E+071,05E-0810,54
45290,8430,5940,0580,7890,0540,5361,4720149259,255,40E+079,93E-099,93
55290,8730,6170,0580,8190,0540,5591,4651162799,255,40E+071,04E-0810,35
65290,8920,6390,0580,8380,0540,5811,4423407928,53,93E+071,48E-0814,78
75290,8650,6140,0580,8110,0540,5561,45863309494,43E+071,26E-0812,55
85290,8960,6330,0580,8420,0540,5751,46434782694,43E+071,30E-0812,98
95290,8830,6240,0580,8290,0540,5661,46466431194,43E+071,28E-0812,78
105290,8610,6110,0580,8070,0540,5531,45931283994,43E+071,25E-0812,48

It was found that the average concentration of the colloidal AuNPs is 11.59 nM. It will be assumed that the fibers have the same concentration


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