User:Allison K. Alix/Notebook/Thesis Research/2013/04/11

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Objectives

  • Complete Gel Electrophoresis on DNA-AuNP hybrid synthesized 3/18
  • Observe under UV light

Procedures

Part 1: Gel Electrophoresis on DNA-AuNP hybrid

The following samples were observed

1) 4μL filtered water + 1μL 100bp DNA ladder + 1μL loading dye

2) 4μL filtered water + 1μL DNA/AuNP + 1μL loading dye

3) 4μL filtered water + 1μL supernatant 1 + 1μL loading dye

4) 4μL filtered water + 1μL supernatant 2 + 1μL loading dye

5) 4μL filtered water + 1μL supernatant 3 + 1μL loading dye

6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye

  • Stain for ~10min in ethidium bromide


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