User:Allison K. Alix/Notebook/Thesis Research/2013/09/10
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Addition of MB
[DNA] = 0.910μM, so [MB] must be at least in 1:1 ratio
[MB] original = 7.61uM
1) Add 20uL 7.61uM MB to 150uL Au/SiO2/DNA solution
[MB] = 0.895uM
[DNA] = 0.802
2) Heat sample for ~20 minutes to stimulate hybridization
3) Cool to room temperature on hot plate
4) Observe absorbance and fluorescence (absorbance peak should be ~532nm)
Fluorescence Spectrum of MB
[Insert Fluor spec]
The Au@SiO@/DNA-MB complex showed an intensity of 80 (relative intensity 0.08) when excited at 532 nm.
FCS Measurements were taken of 10X dilute, 50X diluted, and 100X diluted Au@SiO2/DNA-MB samples. No correlation curve was present.