User:Andrew Yang/Notebook/MURI MIT/2012/02/04

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Workflow for 2/2/2012

1. EX cut J06702 and J85201, ES cut S03119 and Chlor backbone
2. Phosphatase J06702, J85201, and Chlor backbone
3. Make three 1% gels and one 4% gel.
4. Run J06702 EX, J85201 EX, S03119 ES, and Chlor backbone ES on wide cast gels for gel extraction.
5. Run J85201 E, J85201 X, Chlor backbone E, AND Chlor backbone S on analytical to ensure enzymes work.
6. Run annealed J85010, J85012, and J85013 on 4% gel. Run ss oligos (FWD or RVS) to compare.
7. Gel extract J06702 EX, J85201 EX, S03119 ES, and Chlor backbone ES
8. Ligate J85010, J85012, and J85013 with EX cut J06702/J85201, depending on gel results
9. Ligate ES cut S03119 and Chlor backbone
10. Transformations with non-JBabb Turbo cells, if possible.

   10a. Make or find comp cells 

11. Inoculate more J06702 for PCR tomorrow.
12. Miniprep 069








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