User:Andy Maloney/Kinesin & Microtubule Page/K-PEM

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Recipe

Procedure

See the α-PEM procedure for pictures.

  1. Weigh out 25 mg of kappa casein.
  2. Add 25 mL of PEM to the casein.
  3. Place in a beaker with a stir bar and stir for approximately 15-20 minutes on setting 7, or until all the casein is in solution.
  4. Aliquot into vials and store in the 4°C fridge.

Do not filter solution. This casein solution will last for 6 months in the fridge.

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