User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/11/Spin coater 3000 Trial 1

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Trial 1

  1. Place a piece of tape over a glass cover slip.
  2. Spin coat 20 ┬ÁL of 100 mg/mL lipids over the tape covered region and non taped region.
  3. Remove tape.
  4. Place FITC labeled microtubules on both the removed taped region and the non taped region.
  5. Visualize.

There are several things to note about doing this.

  1. You can only use the 18 x 18 mm cover slips.
  2. You have to center the slip on the vacuum chuck.
  3. Setting up flow cells are going to be difficult.

The first several attempts ended up with broken glass. Trying to get tape off of cover slips is tough without breaking them. I've found a good speed to spin the slip at. What that speed is, I have no clue.

After some trial and error, we finally have a sample that may work. You can actually see where the lipid molecules are on the slide. I'm guessing this is due to the passivation having lots of lipid layers on it.

Note It seems to be working. We are definitely seeing microtubules on the non passivated side with stuck ends while on the lipid side, there are none.

Motility assay

I'm just going to try this and see what happens.

Nothing worked...to be explained later.

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