User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/02/Heavy water mapping
The heavy water experiments I am going to do will look at speed effects on gliding microtubules for varying amounts of heavy water in the assay. The heavy water I speak of is D2O in this experiment.
- 0%-"100%" D2O in increments of 10. Also looking at speed affects over time.
The other heavy water I speak of is heavy oxygen water. I have just purchased H218O water from Sigma and since this stuff is so expensive, I plan on doing fewer increments since I am only getting 1g of it to start with. Dr. Koch and I talked and he suggested that this water be placed in the motility solution only. I will probably do the following concentrations.
- 5%, 25%, 75% just to see what happens.
The final water experiment that needs to be done is that for deuterium depleted water. For this one I think I will only do a 5% and a 90(or so)% assay only in the motility solution again.