User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/09/13

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BSA UV-Vis 160913

  1. Samples did not form nanoparticles correctly here.
  2. Samples were remade as described here.
  3. Samples were put into the fluorimeter and analyzed.
  4. Analysis is below.
  1. Photos of the samples before fluorescence analysis are shown below:

This image shows 0.125 mM Fructose, 0.25 mM Au samples with pH 4-12.

This image shows 0.25 mM Fructose, 0.25 mM Au samples with pH 4-12.

Stock Solutions for 160913

  1. AuCl3
    1. mass = 34.62 mg
    2. 25.0 mL volumetric flask
    3. MW = 303.3 g/mol
    4. concentration = 4.57 mM
  2. Bovine Serum Albumin
    1. mass = 35.62 mg
    2. 10.0 mL volumetric flask
    3. MW = 66776 g/mol
    4. concentration = 53.3 mM
  3. Lysozyme
    1. 33.32 mg of lysozyme
    2. 10.0 mL water in volumetric flask
    3. MW = 14307 g/mol
    4. concentration = 232.9 μM
  4. Myoglobin
    1. 32.23 mg of myoglobin
    2. water to 10.0 mL in volumetric flask
    3. MW = 16951 g/mol
    4. concentration = 137 μM

Data 160913

The chart below shows the absorbance scans across 200-800 nm for pH 4-12 with fructose concentration 0.125 mM.

Image:160913 UV 0.125.PNG

The chart below shows the absorbance scans across 200-800 nm for pH 4-12 with fructose concentration 0.25 mM.

Image:160913 UV 0.25.PNG

The chart below shows the A(530) for pH 4-12 with fructose concentration 0.125 mM.

Image:160913 A530 0.125.PNG

The chart below shows the A(530) for pH 4-12 with fructose concentration 0.25 mM.

Image:160913 A530 0.25.PNG

Results 160913

Similarly to the last class, the pH 11-12 solutions were more clear than pH 4-10. They still had much lower absorbances. The pH 4-10 samples had similar peak absorbances across the fluorescence spectrum scan (200-800 nm). The A(530) of pH 4-10 samples was also found to be similar across all samples.

Calculations for tomorrow 160914

  1. Stocks
    1. 4.57 mM Au
    2. 53.3 mM BSA
    3. 0.001 mM HCl
    4. DI Water
  1. Volumes Added to Generate Sample
    1. 175.89 uL BSA
    2. 164.11 uL Au
    3. 30 uL HCl
    4. 2629.99 uL HOH


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