User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/10/11

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Fluorescence Sample Prep 161011

BSA Fluorescence Solution

  1. pH 7
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 49.1 uM
  2. [Au]= 3.73 mM
  3. [HCl]= 1 mM

Volumes for making the solution:

  1. 190.94 uL BSA
  2. 147.64 uL Au
  3. 2661.4 uL HOH

Data Analysis

Image:161011FluorPART1.PNG

Emission maximums of Au NPs at pH 7 over time with [Au]=0.25 mM and [BSA]=3.125 uM.

Image:161011FluorPART2.PNG

Integrated emission of Au NPs at pH 7 over time with [Au]=0.25 mM and [BSA]=3.125 uM.

Image:161011FluorPART3.PNG

Selected scans form 0-1000s of Au NPs at pH 7 over time with [Au]=0.25 mM and [BSA]=3.125 uM.

Image:161011FluorPART4.PNG

Selected scans form 1000-4600s of Au NPs at pH 7 over time with [Au]=0.25 mM and [BSA]=3.125 uM.

Image:161011FluorPART5.PNG

Selected scans form 4600-10800s of Au NPs at pH 7 over time with [Au]=0.25 mM and [BSA]=3.125 uM.



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