User:Anthony Salvagno/Notebook/Research/2011/04/13/pALS PCR Troubleshooting
The temperature in the lab has forced me to lower the temperature on the thermal cycler to 50C for annealing and that produced a PCR product. It also gave me less smear than I typically receive when doing this experiment so that is positive. I will now try to do PCR with labeled reverse primer and unlabeled forward primer and work my way through the tethering construct construction.
That worked... yay! Let's move on to digestion and ligation.